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Vol 43(2009) N 4 p. 626-631; E.R. Surina1, E.V. Morozkina1, A.N. Marchenko1, A.A. Antipin2, O.V. Mitkevich3, V.V. Kushnirov3, M.D. Ter-Avanesyan3, S.V. Benevolensky1 Selection of DNA aptamers specifically interacting with the fibrillar form of the yeast Sup35 protein 1Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, 119071, Russia2State Research Center GosNIIgenetika, Moscow, 113545, Russia 3Russian Cardiology Research and Production Center, Moscow, 121552, Russia Received - 2008-11-19; Accepted - 2008-12-09 Single-stranded DNA aptamers interacting with fibrils of Saccharomyces cerevisiae Sup35p were obtained by the SELEX procedure. The specificity of interactions with Sup35p was investigated for 10 out of 40 selected aptamers. Nine aptamers were found to bind to the fibrillar but not to the monomeric form of Sup35p. The dissociation constant of the aptamer-fibril complex ranged from 0.1 to 1.0 μM for different aptamers. The aptamers can be used to study the prion transformation of Sup35p. myloid fibril, aptamer, prion, SELEX, Sup35, yeast |
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