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Vol 57(2023) N 3 p. 424-439; DOI 10.1134/S002689332303010X  A.N. Sinyakov1*, E.V. Kostina1 DNA Fragment Enrichment for High-Throughput Sequencing 1Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090 Russia*sinyakov@niboch.nsc.ru Received - 2022-11-08; Revised - 2022-12-16; Accepted - 2022-12-16 This review describes the application of oligonucleotides, which are mainly obtained using DNA synthesizers of a new generation (microarray DNA synthesizers), for the enrichment of target genomic fragments. The methods of molecular hybridization, polymerase chain reaction, and CRISPR-Cas9 system for this purpose are considered. Examples of the practical use of the developed methods for research and diagnostic purposes are given. high-throughput sequencing, oligonucleotides, microarray, amplification, diagnostics, exome, CRISPR-Cas |
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