|
Vol 54(2020) N 3 p. 416-420; DOI 10.1134/S0026893320030036 K.I. Adameyko1, O.I. Kravchuk1*, A.D. Finoshin1, A.N. Bonchuk2, A.A. Georgiev3, V.S. Mikhailov1, N.G. Gornostaev1, K.V. Mikhailov3,4, A.V. Bacheva3, M.I. Indeykina5, A.E. Bugrova5, G.R. Gazizova6, O.S. Kozlova6, O.A. Gusev6,7, E.I. Shagimardanova6, Y.V. Lyupina1 Structure of Neuroglobin from Cold-Water Sponge Halisarca dujardinii 1Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, 119334 Russia2Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia 3Moscow State University, Moscow, 119991 Russia 4Kharkevich Institute for Information Transmission Problems, Russian Academy of Sciences, Moscow, 127051 Russia 5Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, 119334 Russia 6Kazan Federal University, Kazan, 420008 Russia 7KFU-RIKEN Translational Genomics Unit, RIKEN National Science Institute, Yokohama, 230-0045 Japan *kravchuk444@mail.ru Received - 2019-10-23; Revised - 2019-11-22; Accepted - 2019-12-02 The iron-containing protein neuroglobin (Ngb) involved in the transport of oxygen is generally considered the precursor of all animal globins. In this report, we studied the structure of Ngb of the cold-water sponge Halisarca dujardinii. In sponges, the oldest multicellular organisms, the Ngb gene contains three introns. In contrast to human Ngb, its promoter contains a TATA-box, rather than CG-rich motifs. In sponges, Ngb consists of 169 amino acids showing rather low similarity with its mammalian orthologues. It lacks Glu and Arg residues in positions required for prevention of hypoxia-related apoptosis. Nevertheless, Ngb contains both proximal and distal conserved heme-biding histidines. The primary structure of H. dujardinii neuroglobin predicted by sequencing was confirmed by mass-spectrometry analysis of recombinant Ngb expressed in E. coli. The high level of Ngb expression in sponge tissues suggests its possible involvement in the gas metabolism and presumably in other key metabolic processes in H. dujardinii. neuroglobin, Halisarca dujardinii, gene structure |