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Vol 43(2009) N 1 p. 1-7; Qin Gui1, Jialu Wang2, Yanhao Xu1, Jianbo Wang1 Expression changes of duplicated genes in allotetraploids ofBrassica detected by SRAP-cDNA technique 1Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China2Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, 100084, China Received - 2008-01-09; Accepted - 2008-03-18 Polyploidization plays an important role in plant evolution, and it may trigger changes in genome structure and gene expression. The purpose of this study was to detect the extent of gene expression alterations in natural Brassica allotetraploids. Sequence-related amplified polymorphism (SRAP)-cDNA display was carried out on six species (13 accessions), three allotetraploids and their three ancestral diploids. Total of 1496 transcripts were screened, finding that 92 (6.1%) transcripts were subjected to silencing in allopolyploids, while 19 (1.3%) transcripts were activated. The results indicated that a significant fraction of duplicated genes have shown expression alterations in the natural allotetraploids compared with their diploid parents. Brassica, gene silencing, polyploidy, SRAP-cDNA |
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