2017  0,977
2016  0,799
2015  0,662
2014  0,740
2013  0,739
2012  0,637
2011  0,658
2010  0,654
2009  0,570
2008  0,849
2007  0,805
2006  0,330
2005  0,435
2004  0,623
2003  0,567
2002  0,641
2001  0,490
2000  0,477
1999  0,762
1998  0,785
1997  0,507
1996  0,518
1995  0,502
Vol 52(2018) N 6 p. 878-890; DOI 10.1134/S002689331806002X Full Text

N.V. Andreeva1, K.V. Zotov2, Y.Y. Yegorov1, O.F. Kandarakov3, V.I. Yusupov2, A.V. Belyavsky1*

Cytotoxic Effect of Low-Intensity Infrared Laser Irradiation on Human Melanoma Cells

1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia
2Institute of Photon Technologies, Federal Research Center of Crystallography and Photonics, Russian Academy of Sciences, Moscow, Troitsk, 108840 Russia
3Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation, Moscow, 115478 Russia

Received - 2017-11-20; Accepted - 2017-12-12

Continuous low-intensity laser irradiation (LILI) affects the state of cells in culture, including their proliferation rate. Data collected with various cell models vary significantly, but most studies have reported positive effects of LILI on cell proliferation. The effects of continuous infrared LILI (835 nm) was studied using three independent different melanoma cell lines. The LILI effect was shown to strongly depend on the irradiation dose. Higher doses (230 kJ/m2) significantly suppressed the cell growth. A further increase in LILI dose led to a significant cytotoxic effect, which increased disproportionately quickly with the increasing light intensity. Human mesenchymal stem cells (MSCs) were found to be significantly more resistant to the cytotoxic effect of higher-dose LILI. Importantly, the effects were not due to the difference in culture conditions. Control experiments showed that 15 non-melanoma tumor cell lines were more resistant to LILI than melanoma cells. Selective sensitivity of melanoma cells to LILI in vitro was assumed to provide a basis for LILI-based approaches to melanoma treatment.

melanoma, lasers, infrared low-intensity laser irradiation, mesenchymal stem cells, cytotoxicity