Vol 52(2018) N 4 p. 548-555; DOI 10.1134/S0026893318040039
V.I. Butvilovskaya1*, O.V. Smoldovskaya1, G.U. Feyzkhanova1 , M.A. Filippova1, L.V. Pavlushkina2, S.A. Voloshin1, A.Yu. Rubina1
Modification of Anti-Glycan IgG and IgM Profiles in Allergic Inflammation1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia
2Filatov Moscow Pediatric Clinic no. 13, Healthcare Department of Moscow, Moscow, 123001 Russia
Received - 2017-11-15; Accepted - 2017-11-29
Glycans and anti-glycan antibodies (AGAs) are essential for infiltration of inflammatory cells in various allergies. The glycocalyx structure of the cells is modified during disease progression, and this modification is possible to evaluate by assessment of AGAs. A printed glycan array with 55 immobilized glycans and immobilized antibodies to IgG, IgA, and IgM was used to study the changes in AGA profiles in bronchial asthma (BA). Levels of antibodies to certain glycans in BA patients statistically differed from levels in healthy donors (p < 0.0007 by the Mann-Whitney test); the glycan set included 6Su-6`-SiaLec, Sia LeX, Sia6Htype2; Tαα, Manβ1-4GlcNAc, and Manα1-4Manβ. The obtained results help to better understand the mechanisms of the cell-mediated immune response in bronchial asthma and other types of allergic reactions.
allergy, bronchial asthma, anti-glycan antibodies, glycan array, immune response, siglecs, galectins