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Vol 52(2018) N 3 p. 414-418; DOI 10.1134/S0026893318030081 Full Text

A.V. Kudryavtseva1,2*, K.M. Nyushko2, A.R. Zaretsky3,4, D.A. Shagin3, A.F. Sadritdinova1,2, M.S. Fedorova1, M.V. Savvateeva1, Z.G. Guvatova1, E.A. Pudova1, B.Y. Alekseev2, A.A. Dmitriev1, A.V. Snezhkina1

Suppression of NR0B2 gene in Clear Cell Renal Cell Carcinoma Is Associated with Hypermethylation of Its Promoter

1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia
2National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, 125284 Russia
3Pirogov Russian National Research Medical University, Moscow, 117997 Russia
4Evrogen Lab LCC, Moscow, 117437 Russia

*rhizamoeba@mail.ru
Received - 2017-05-11; Accepted - 2017-07-12

Clear cell renal cell carcinoma (ccRCC) is a common urologic malignancy. Understanding of the transcriptional regulation of oncogenes and tumor suppressor genes involved is critical for the development of the treatments for renal tumors. Using ccRCC subdivision of the TCGA dataset, we identified NR0B2 encoding orphan nuclear receptor as a tumor suppressor candidate in renal tissue. In independent cohort of primary renal tumors, quantitative PCR experiments confirmed significant suppression of NR0B2 mRNA in 86% of ccRCC samples studied. In 80% of these cases, we detected the hypermethylation of the NR0B2 pro-moter region. These results suggest that NR0B2 is a tumor suppressor gene in ccRCC, and that the hypermethylation of promoter region is the main mechanism of its downregulation.

clear cell renal cell carcinoma, NR0B2, quantitative PCR, bisulfite sequencing, tumor suppressor gene, DNA methylation



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