Vol 52(2018) N 1 p. 19-22; DOI 10.1134/S002689331801017X
P.A. Sakharov, S.Ch. Agalarov*
Mutant Initiation Factor eIF4A (R362Q) Does Not Suppress the Assembly of the 48S Preinitiation Complex on mRNA with the Leader Sequence of mRNA That Encodes for ObelinInstitute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290 Russia
Received - 2017-05-31; Accepted - 2017-07-05
The formation of ribosomal 48S initiation complexes at the start codon of the mRNA leader sequence that encodes obelin has been studied using the method of primer extension inhibition (toeprinting). Experiments have been performed in a system composed of purified individual components required to initiate translation. The influence of the dominant negative mutant of factor eIF4A (R362Q) on translation initiation has been studied. It has been shown that the presence of the mutant in reaction mixture has no effect on efficiency of formation of the 48S complexes at start codon of the template studied.
translation initiation, uncapped mRNA, initiation factors eIF4A and eIF4F, mutant factor eIF4A (R362Q)