2017  0,977
2016  0,799
2015  0,662
2014  0,740
2013  0,739
2012  0,637
2011  0,658
2010  0,654
2009  0,570
2008  0,849
2007  0,805
2006  0,330
2005  0,435
2004  0,623
2003  0,567
2002  0,641
2001  0,490
2000  0,477
1999  0,762
1998  0,785
1997  0,507
1996  0,518
1995  0,502
Vol 51(2017) N 5 p. 712-717; DOI 10.1134/S0026893317050053 Full Text

V.I. Butvilovskaya1*, A.A. Tikhonov1, E.N. Savvateeva1, A.A. Ragimov2, E.L. Salimov2, S.A. Voloshin1, D.V. Sidorov3, M.A. Chernichenko3, A.P. Polyakov3, M.M. Filushin3, M.V. Tsybulskaya1, A.Yu. Rubina1

Hydrogel microchip as a tool for studying exosomes in human serum

1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia
2Sechenov First Moscow State Medical University, Moscow, 119991 Russia
3Herzen Moscow Cancer Research Institute, Ministry of Healthcare of the Russian Federation, Moscow, 125284 Russia

Received - 2016-12-14; Accepted - 2016-12-26

Exosomes are cell-derived vesicles that are secreted by both normal and cancer cells. Over the last decade, a few studies have revealed that exosomes cross talk and/or influence major tumor-related pathways such as angiogenesis and metastasis involving many cell types within the tumor microenvironment. The protein composition of the membrane of an exosome reflects that of the membrane of the cell of origin. Because of this, tumor-derived exosomes differ from exosomes that are derived from normal cells. The detection of tumor exosomes and analysis of their molecular composition hold promise for diagnosis and prognosis of cancer. Here, we present hydrogel microarrays (biochips), which contain a panel of immobilized antibodies that recognize tetraspanins (CD9, CD63, CD81) and prognostic markers for colorectal cancer (A33, CD147). These biochips make it possible to analyze the surface proteins of either isolated exosomes or exosomes that are present in the serum samples without isolation. These biochips were successfully used to analyze the surface proteins of exosomes from serum that was collected from a colorectal cancer patient and healthy donor. Biochip-guided immunofluorescent analysis of the exosomes has made it possible for us to detect the A33 antigen and CD147 in the serum sample of the colorectal cancer patient with normal levels of CEA and CA19-9.

Hydrogel, exosomes, microvesicles, CD antigen, tetraspanins, exosome detection