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Vol 45(2011) N 3 p. 479-483; O.E. Melkina, V.Y. Kotova, I.V. Manukhov, G.B. Zavilgelsky* Effects of the IbpAB and ClpA Chaperones on DnaKJE-Dependent Refolding of Bacterial Luciferases in Escherichia coli Cells State Research Center GosNIIGenetika, Moscow, 117545 Russia*zavilgel@genetika.ru Received - 2010-10-22; Accepted - 2010-11-16 Rate and level of DnaKJE-dependent refolding of the thermoinactivated Aliivibrio fischeri luciferase are considerably lower in Escherichia coli ibpA and ibpB, clpA mutants than in wild type cells. The rate and level of refolding are lower in E. coli ibpB::kan than in ibpA::kan cells. The decline of refolding level in E. coli clpA::kan makes progress only with the increase of thermoinactivation time of luciferase. Plasmids with genes ibpAB do not compensate clpA mutation. It is supposed that small chaperones IpbAB and chaperone ClpA operate independently in a process of DnaKJE-dependent refolding of proteins at the different stages. chaperone, IbpAB, ClpA, DnaKJE, refolding, luciferases |
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