2014  0,718
2013  0,739
2012  0,637
2011  0,658
2010  0,654
2009  0,570
2008  0,849
2007  0,805
2006  0,330
2005  0,435
2004  0,623
2003  0,567
2002  0,641
2001  0,490
2000  0,477
1999  0,762
1998  0,785
1997  0,507
1996  0,518
1995  0,502
Vol 51(2017) N 2 p. 269-273; DOI 10.1134/S0026893317010022 Full Text

N. V. Aleksandrova1*, E.S. Shubina1, A.N. Ekimov1, T.A. Kodyleva1, I.S. Mukosey1, N.P. Makarova1, E.V. Kulakova1, L.A. Levkov1, I.Yu. Barkov1, D.Yu. Trofimov1, G.T. Sukhikh1

Comparative results of preimplantation genetic screening by array comparative genomic hybridization and new-generation sequencing

1Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation, Moscow, 117997 Russia

Received - 2016-03-17; Accepted - 2016-06-01

Aneuploidies as quantitative chromosome abnormalities are a main cause of failed development of morphologically normal embryos, implantation failures, and early reproductive losses. Preimplantation genetic screening (PGS) allows a preselection of embryos with a normal karyotype, thus increasing the implantation rate and reducing the frequency of early pregnancy loss after IVF. Modern PGS technologies are based on a genome-wide analysis of the embryo. The first pilot study in Russia was performed to assess the possibility of using semiconductor new-generation sequencing (NGS) as a PGS method. NGS data were collected for 38 biopsied embryos and compared with the data from array comparative genomic hybridization (array-CGH). The concordance between the NGS and array-CGH data was 94.8%. Two samples showed the karyotype 47,XXY by array-CGH and a normal karyotype by NGS. The discrepancies may be explained by loss of efficiency of array-CGH amplicon labeling.

preimplantation genetic screening, preimplantation genetic diagnosis, aneuploidy, assisted reproductive technologies, IVF, embryo biopsy, array comparative genomic hybridization (aCGH), next-generation sequencing (NGS)