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Vol 51(2017) N 2 p. 333-338; DOI 10.1134/S0026893317010149 Full Text

A.A. Pulkina1,2, M.V. Sergeeva1*, S.V. Petrov1, A.V. Fadeev1, A.B. Komissarov1, E.A. Romanovskaya-Romanko1, M.V. Potapchuk1, L.M. Tsybalova1

Impact of mutations in nucleoprotein on replication of influenza virus A/Hong Kong/1/68/162/35 reassortants at different temperatures

1Research Institute of Influenza WHO National Influenza Centre of Russia, St. Petersburg, 197376 Russia
2Peter the Great St. Petersburg Polytechnic University, Saint-Petersburg, 195251 Russia

*mari.v.sergeeva@gmail.com
Received - 2016-04-15; Accepted - 2016-05-19

The nucleoprotein (NP) of influenza virus is a multifunctional RNA binding protein. The role of NP in the adaptation of influenza viruses to a host has been experimentally proved. Ambiguous data are available on the role of nucleoprotein in the attenuation of influenza A viruses, which is characterized by ability to replicate at low temperature (26°C) and inability to replicate at high temperature (39°C). Influenza virus donor strain A/Hong Kong/1/68/162/35 (H3N2), adapted to growth at low temperature, differs from the wild type virus by 14 amino acid mutations in the internal and non-structural proteins. Two mutations occurred in the NP: Gly102Arg and Glu292Gly. We have obtained viruses with point reverse-mutations in these positions and compared their replication at different temperatures by measuring infectious activity in chicken embryos. It has been shown that reverse mutation Gly292Glu in the NP reduced virus ability to replicate at low temperature, the introduction of the second reverse mutation Arg102Gly completely abolished virus cold adaptation.

influenza virus nucleoprotein, reverse-mutation, cold adapted phenotype, temperature sensitive phenotype, reassortant



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