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Vol 44(2010) N 5 p. 748-753; C.R. Wang1*, D.Y. Hou2, H.G. Feng3, B.S. Yang3, C.S. Xu4, J.T. Lin3,5,6 Induction of new ADAM related proteins from treated human chang-liver cells 1Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, 453003, China2College of agriculture, Henan University of Science And Technology, Luoyang, 471003, China 3Department of Life Science and Technology, Xinxiang Medical University, Xinxiang, 453003, China 4Co-Construction Key Laboratory of Cell Differentiation and Regulation, Henan Normal University, Xinxiang, 453007, China 5Key Open Laboratory for Tissue Regeneration of Henan Universities, Xinxiang Medical University, Xinxiang, 453003, China 6Institute of Anatomy I, School of Medicine, University of Jena, Jena, 07743, Germany *juntang.lin@googlemail.com Received - 2009-09-03; Accepted - 2009-12-14 Chang-liver cells is a cell line generated from human liver tissue, which is often used in scientific research. ADAMs are a family of proteins that consist of multi-domains, possess multi-functions and play a central role in normal or abnormal physiological conditions, such as regeneration and tumorigenesis. To investigate the expression and functional alteration of the ADAMs or ADAM related proteins in Chang-liver cells, this cell line was treated with heat stress, modified Hanks solution containing ATP or other buffers. Our results showed that the treatment with Hanks solution containing ATP induces Chang-liver cells to express new ADAM related proteins. To analyze these new ADAM related proteins, a cDNA expression library was constructed for the treated Chang-liver cells. A series of positive clones were obtained through immunoscreening with an ADAMs common antibody. A new ADAM related protein possessing alkaline protease activity was confirmed in these clones Chang-liver cell, non-nutrition medium, ADAMs, ADAM related protein, immunoscreening, alkaline protease |
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