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Vol 44(2010) N 2 p. 334-340;
Y.A. Bukurova1, S.L. Hankin2, G.S. Krasnov1, E.S. Grigor'eva3, T.D. Mashkova1, N.A. Lisitsyn1, V.L. Karpov1, S.F. Beresten1*

Estimation of the Efficiency of 2D Analysis and Bioinformatics Search in Identification of Protein Markers for Colon Tumors

1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia
2Medical Center of the Bank of Russia, Moscow, 117593 Russia
3Cancer Research Institute, Siberian Branch, Russian Academy of Medical Sciences, Tomsk, 634009 Russia

*sberesten@gmail.com
Received - 2009-07-09; Accepted - 2009-07-30

A modification of proteome differential analysis was developed; it comprises initial removal of the cell structural proteins by extraction with buffered saline and protein fractionation by gel filtration with subsequent separation by two-dimensional gel electrophoresis and identification by mass-spectrometry. This approach provided for detection of 12 proteins with significantly elevated expression levels in the majority of the analyzed malignant colorectal tumor specimens as compared with normal tissues. Increased contents of eight proteins were discovered for the first time. The efficiencies of the search for tumor markers by 2D analysis and serial analysis of gene expression (SAGE) were compared using the control panel of 19 potential colorectal tumor markers, identified earlier or independently found by other researchers. The 2D data for the control panel matched the earlier published data, whereas the search of SAGE database succeeded in detection only one-third of the markers.

colorectal cancer, two-dimensional gel electrophoresis, bioinformatics proteomics, mass spectrometry



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