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Vol 44(2010) N 1 p. 67-76;
D.A. Mogilenko1,2*, I.V. Kudrjavtsev2, S.V. Orlov1,2, A.D. Kharazova1,2, A.V. Polevshikov1,2

Expression of the Starfish Complement Component C3 Gene Homolog under the Influence of Bacterial Lipopolysaccharide

1Shanghai Key Laboratory of Veterinary Biotechnology, , School of Agriculture and Biology, Shanghai Jiao Tong University, shanghai, 200240, China
2Key Laboratory of Animal Disease Diagnosis and Immunology of Ministry of Agriculture,Nanjing Agricultural University , Nanjing, 210095, China

*denis@iem.sp.ru
Received - 2009-03-25; Accepted - 2009-05-13

A fragment of a homolog of the complement component C3 gene was cloned and sequenced from the starfish Asterias rubens. A phylogenetic analysis of the gene, termed ArC3-like, demonstrated its close similarity to the C3 gene homolog of deuterostome invertebrates. A high level of ArC3-like mRNA expression was observed in circulating cells (coelomocytes), a gut derivate (hepatopancreas), and the male gonad, but not in the stomach, female gonad, and rectal gland of A. rubens. ArC3-like gene expression was detected in all types of starfish coelomocytes, including lymphocyte-like cells and granular and nongranular amebocytes. Bacterial lipopolysaccharide (LPS) injected into the coelomic cavity of starfish increased the ArC3-like gene expression in coelomocytes and the hepatopancreas as compared to the control level (sterile sea water injection). The level of ArC3-like gene expression in coelomocytes in response to LPS reached its maximum 6 h after the injection and decreased to the baseline level 24 h after the injection. In the hepatopancreas, the level of ArC3-like gene expression reached its maximum 6--12 h after the LPS injection stimulation and remained high even 48 h after the injection. A long-term upregulation in response to LPS was demonstrated for the ArC3-like gene.

innate immunity, echinoderms, starfish, complement component C3, coelomocytes, LPS



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