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Vol 50(2016) N 2 p. 302-306; DOI 10.1134/S0026893316020035 Full Text

K.M. Burnysheva, I.Yu. Petrushanko, P.V. Spirin, V.S. Prassolov, A.A. Makarov, V.A. Mitkevich*

Ribonuclease binase induces death in T-cell acute lymphoblastic leukemia cells by apoptosis

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia

*mitkevich@gmail.com
Received - 2015-07-22; Accepted - 2015-10-05

Bacterial ribonuclease binase is a potential anticancer agent. In the present study, we have determined the toxic effect of binase towards cell lines of T-cell acute lymphoblastic leukemia Jurkat and CEMss. We have shown that binase induces apoptosis in these cells. At the same time, binase does not cause toxic effects in leukocytes of healthy donors, which suggests that binase activity towards leukemic cells is selective. We have found that the treatment of cancer cells with binase leads to a reduction in reactive oxygen species and transcription factor NFκB levels, and demonstrated that these effects are a common feature of the action of RNases on cancer cells.

Ribonuclease, cytotoxicity, apoptosis, reactive oxygen species, mitochondrial membrane potential, transcription factor NFκB



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