JMB-HEADER RAS-JOURNALS EIMB Pleiades Publishing

RUS

             

ENG

YearIMPACT-FACTOR
2022  1,200
2021  1,540
2020  1,374
2019  1,023
2018  0,932
2017  0,977
2016  0,799
2015  0,662
2014  0,740
2013  0,739
2012  0,637
2011  0,658
2010  0,654
2009  0,570
2008  0,849
2007  0,805
2006  0,330
2005  0,435
2004  0,623
2003  0,567
2002  0,641
2001  0,490
2000  0,477
1999  0,762
1998  0,785
1997  0,507
1996  0,518
1995  0,502
Vol 42(2008) N 3 p. 463-468;
D.S. Karpov, V.V. Tyutyaeva, S.F. Beresten, V.L. Karpov

Mapping of the Rpn4p regions responsible for transcriptional activation of proteasome genes

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia
Received - 2008-01-28; Accepted - 2008-01-29

Rpn4p is an extremely short-lived proteasome-associated protein that acts both as a positive and negative transcriptional regulator of the ubiquitin-proteasome system and as its substrate. The mechanisms of proteasomal degradation of Rpn4p have been studied in great detail; however, the mechanisms of its own action remain unclear and, first of all, its functional domains are unknown. To map the functionally important regions, a set of Rpn4p deletion derivates was constructed. The mutant proteins were expressed in Saccharomyces cerevisiae strain rpn4-Δ, their contents were determined by Western blotting, and their activity was assessed by measuring the mRNA levels of proteasome genes. Deletions of the C-terminal region, containing DNA-binding zinc finger domains, and the N-terminal region, having no homology to the transactivation domains of any known transcription factor, completely inactivated Rpn4p. Only one of the two acidic regions, putative transactivation domains, proved to participate in transcriptional activation. Deletions of the N-terminal region, NAD, or zinc finger domains rendered Rpn4p metabolically stable. These data provide new insights into the mechanisms of the Rpn4p functioning and degradation.

Rpn4p, deletion analysis, semiquantitative RT-PCR, gene expression, transcriptional regulation, proteasome, Saccharomyces cerevisiae



JMB-FOOTER RAS-JOURNALS