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Vol 42(2008) N 3 p. 381-390;
Keji Jiang1, Zhihua Liao2, Yan Pi1, Zhuoshi Huang1, Rong Hou1, Ying Cao1, Qing Wang1, Xiaofen Sun1, Kexuan Tang1,3

Molecular cloning and expression profile of a jasmonate biosynthetic pathway gene for allene oxide cyclase from Hyoscyamus niger

1State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R & D Center, Morgan-Tan International Center for Life Sciences, Fudan University, Shanghai, 200433, People's Republic of China
2Laboratory of Natural Products and Metabolic Engineering, Chongqing Sweet Potato Research Center, Key Laboratory of Eco-environments in Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing, 400715, People's Republic of China
3Plant Biotechnology Research Center, School of Agriculture and Biology, Fudan-SJTU-Nottingham Plant Biotechnology R & D Center, Shanghai Jiao Tong University, Shanghai, 200030, People's Republic of China
Received - 2007-05-18; Accepted - 2007-06-16

Hyoscyamus niger L. is a medicinal plant which produces a class of jasmonate-responsive pharmaceutical secondary metabolites named tropane alkaloids. As a family of signaling phytohormones, jasmonates play significant roles in the biosynthesis of many plant secondary metabolites. In the jasmonate biosynthetic pathway of plants, allene oxide cyclase (AOC, EC 5.3.99.6) catalyzes the most important step. Here we cloned a cDNA from H. niger, named HnAOC (GenBank accession no.: AY708383), which was 1044 bp long, with a 747-bp open reading frame (ORF) encoding a polypeptide of 248 amino acid residues. Southern blot analysis indicated that it was a multicopy gene. RT-PCR analysis revealed that the expression of HnAOC was regulated by various stresses and elicitors, with methyl-jasmonate showing the most prominent inducement. The characterization of HnAOC would be helpful for improving the production of valuable secondary metabolites by regulating the biosynthesis of jasmonates.

allene oxide cyclase, Hyoscyamus niger, jasmonate, RT-PCR, secondary metabolite



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