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Vol 49(2015) N 6 p. 899-903; DOI 10.1134/S0026893315060229  E.S. Ioudinkova1*, A.V. Nefedochkina2, O.V. Iarovaia1, S.V. Razin1,2 Detection of complementary transcripts for the intergenic region of the chicken α-globin gene domain 1Institute of Gene Biology, Russian Academy of Sciences, Moscow, 117334;2Biological Faculty, Moscow State University, Moscow, 119992 Russia *ioudinkova@inbox.ru Received - 2015-03-04; Accepted - 2015-03-24 Strand-specific reverse transcription with specific primers and subsequent quantitative real-time PCR with TaqMan probes were used to characterize the transcription profile for both strands of a chicken α-globin gene domain region that harbors the embryonic gene π, the adult gene αD, and a spacer region between them. Complementary transcripts of the spacer region were observed in erythroid cells of the adult lineage. The observation implicates RNA interference in controlling the switch in α-globin gene expression. α-globin gene domain, regulation of transcription, gene expression |
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