JMB-HEADER RAS-JOURNALS EIMB Pleiades Publishing

RUS

             

ENG

YearIMPACT-FACTOR
2022  1,200
2021  1,540
2020  1,374
2019  1,023
2018  0,932
2017  0,977
2016  0,799
2015  0,662
2014  0,740
2013  0,739
2012  0,637
2011  0,658
2010  0,654
2009  0,570
2008  0,849
2007  0,805
2006  0,330
2005  0,435
2004  0,623
2003  0,567
2002  0,641
2001  0,490
2000  0,477
1999  0,762
1998  0,785
1997  0,507
1996  0,518
1995  0,502
Vol 47(2013) N 2 p. 293-298;
K.P. Fedorova1*, I.S. Scharafutdinov1, E.Y. Turbina2, M.I. Bogachev3, O.N. Ilinskaya1, A.R. Kayumov1

C-Terminus of Transcription Factor TnrA from Bacillus subtilis Controls DNA-Binding Domain Activity but Is Not Required for Dimerization

1Kazan (Volga region) federal university, Kazan, 420008 Russia
2Voronezh State University, Voronezh, 394006 Russia
3Saint-Petersburg Electrotechnical University «LETI», Saint-Petersburg, 197376 Russia

*kpfedorova@gmail.com
Received - 2012-08-28; Accepted - 2012-10-02

The transcription factor TnrA, which belongs to the MerR transcription regulators, in Bacillus subtilis controls genes of nitrogen metabolism during nitrogen limiting conditions. As all the DNA-binding proteins, it is active as a dimer in cells, but the dimerization site is still unknown. The multiple alignment of TnrA homologs from other Bacilli allowed to identify the putative dimerization sites. Using the C-terminal truncated TnrA proteins it is established, that, in contrast to other MerR-proteins, the TnrA C-terminus does not participate in dimerization. Surface plasmon resonance has revealed that C-terminus truncations of TnrA do not inactivate its DNA-binding activity. Contrary, increased the affinity towards DNA, confirming that C-terminus controls the DNA-binding activity in a full-length TnrA.

Bacillus subtilis, transcription factor TnrA, dimerization



JMB-FOOTER RAS-JOURNALS