JMB-HEADER RAS-JOURNALS EIMB Pleiades Publishing

RUS

             

ENG

YearIMPACT-FACTOR
2022  1,200
2021  1,540
2020  1,374
2019  1,023
2018  0,932
2017  0,977
2016  0,799
2015  0,662
2014  0,740
2013  0,739
2012  0,637
2011  0,658
2010  0,654
2009  0,570
2008  0,849
2007  0,805
2006  0,330
2005  0,435
2004  0,623
2003  0,567
2002  0,641
2001  0,490
2000  0,477
1999  0,762
1998  0,785
1997  0,507
1996  0,518
1995  0,502
Vol 46(2012) N 5 p. 678-686;
V.V. Kolobov1*, T.V. Davydova2, I.A. Zakharova2, V.Y. Gorbatov2, V.G. Fomina2

Glutamate Antibodies Repress Expression of Dffb Gene in Brain of Rats in Experimental Alzheimer s Disease

1Scientific Centre of Neurology of RAMS, Moscow, 125367 Russia
2Institute of General Pathology and Pathophysiology of RAMS, Moscow, 125315 Russia


*f.neurochemistry@gmail.com
Received - 2011-11-13; Accepted - 2012-02-10

The rat model of Alzheimer's disease including injection of neurotoxic fragment of β-amyloid protein Аβ25-35 into giant-cell nuclei basalis of Meynert was used for experiments. We have investigated the influence of glutamate antibodies administered intranasally in a dose of 300 чg/kg after 1 h of the mentioned alteration on the level of expression of Dffb mRNA. Dffb gene codes caspase-dependent DNase, which participates in the internucleosomal fragmentation of genome DNA during apoptosis. On the third day after the injection of Аβ25-35, we obtained a significant decrease in Dffb gene expression in the prefrontal cortex (37% decrease) and hippocampus (62% decrease) in the group of experimental animals compared to the control group. In the hypothalamus, there were no such differences. Seemingly, the repressing action of glutamate antibodies on the mRNA expression of the Dffb gene reflects the stabilization of processes that take place in the brain cells during experimental Alzheimer's disease; meanwhile, the intensity of the apoptotic death of neurons and glial cells decreases.

expression level, Dffb, glutamate antibodies, experimental Alzheimer's disease, prefrontal cortex, hippocampus, hypothalamus, real-time polymerase chain reaction



JMB-FOOTER RAS-JOURNALS