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Vol 59(2025) N 4 p. 483-493; DOI 10.1134/S002689332570013X  A.N. Sinyakov1*, E.V. Kostina1 Error Correction Techniques in Synthetic Oligonucleotides and Synthetic DNA 1Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090 Russia*sinyakov@niboch.nsc.ru Received - 2024-08-29; Revised - 2024-12-24; Accepted - 2025-02-06 Methods for correcting errors in synthetic oligonucleotides and genetic constructs derived from them are described. Methods for isolating oligonucleotides with a perfect structure from oligonucleotide pools are discussed. Methods for correcting errors in the DNA structure using mismatch-specific endonucleases and proteins of the bacterial DNA repair system are considered. Examples of practical application of the methods developed for adjusting the structure of synthesized genetic constructs are given. oligonucleotides, hybridization, NGS, mismatch-specific endonucleases, MutH, MutL, MutS, synthetic DNA |
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